Composite

Part:BBa_K1116004:Design

Designed by: Shuguang Peng   Group: iGEM13_Tsinghua-A   (2013-09-11)


CAG-EYFP-miRNA-FF3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1791
    Illegal EcoRI site found at 2806
    Illegal EcoRI site found at 2961
    Illegal XbaI site found at 1695
    Illegal XbaI site found at 3004
    Illegal PstI site found at 2046
    Illegal PstI site found at 2966
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1791
    Illegal EcoRI site found at 2806
    Illegal EcoRI site found at 2961
    Illegal PstI site found at 2046
    Illegal PstI site found at 2966
    Illegal NotI site found at 2562
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1791
    Illegal EcoRI site found at 2806
    Illegal EcoRI site found at 2961
    Illegal BamHI site found at 698
    Illegal BamHI site found at 2992
    Illegal XhoI site found at 2703
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1791
    Illegal EcoRI site found at 2806
    Illegal EcoRI site found at 2961
    Illegal XbaI site found at 1695
    Illegal XbaI site found at 3004
    Illegal PstI site found at 2046
    Illegal PstI site found at 2966
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1791
    Illegal EcoRI site found at 2806
    Illegal EcoRI site found at 2961
    Illegal XbaI site found at 1695
    Illegal XbaI site found at 3004
    Illegal PstI site found at 2046
    Illegal PstI site found at 2966
    Illegal NgoMIV site found at 1383
    Illegal NgoMIV site found at 1540
    Illegal AgeI site found at 1829
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

It comes from plasmids of Xie Lab.

References